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Remove medium, and rinse with 0.25% trypsin, 0.53% EDTA solution (ATCC ® 30-2101™). Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.Neodymium Series - Pro Audio | Eminence SpeakerThe basic design of the loudspeaker hasn''t changed much in the past 50 years. On the contrary, many materials incorporated in loudspeaker design have improved dramatically. Back in the 50s and 60s, Alnico was the most common magnet used in loudspeakers. However, a shortage in materials and increased costs for Alnico during that time ...

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The Fern Denzong Hotel & Spa, Gangtok

The Fern Denzong Hotel offers 70 well-furnished rooms with mesmerizing mountain views from the hotel rooftop. Guests can choose from the Winter Green (Avg 260 Sq. Ft.), Winter Green Premium (Avg 285 Sq. Ft.), Fern Club (430 Sq. Ft.) and the Hazel Suite (560 Sq. Ft.) which comes with an attached living room.Records | Twenty20 Internationals | Fielding records ...Read about Records, /, Twenty20 Internationals, /, Most catches Cricket Team Records only on ESPNcricinfo . Check the Stats & Records of Records, /, Twenty20 Internationals, /, Most catches Players in Wicket keeper Batting Bowling

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